Calcium Imaging Datasets
- Calcium imaging videos from human pancreas slices at the Univerity of Florida 2018-2024 https://zenodo.org/records/14605547
- Related paper: Huber MK, Widener AE, Cuaycal AE, Smurlick D, Butterworth EA, Lenchik NI, Chen J, Beery M, Hiller H, Verney E, Kusmartseva I, Rupnik MS, Campbell-Thompson M, Gerling IC, Atkinson MA, Mathews CE, Phelps EA. Beta cell dysfunction occurs independently of insulitis in type 1 diabetes pathogenesis. Cell Rep. 2025 Sep 23;44(9):116174. doi: 10.1016/j.celrep.2025.116174. Epub 2025 Aug 27. PMID: 40875294; PMCID: PMC12550822.
- Slice calcium imaging data analysis code https://github.com/PhelpsLabUF/Slice_Calcium_Imaging_Visualization_Analysis
- Related paper: Huber MK, Widener AE, Cuaycal AE, Smurlick D, Butterworth EA, Lenchik NI, Chen J, Beery M, Hiller H, Verney E, Kusmartseva I, Rupnik MS, Campbell-Thompson M, Gerling IC, Atkinson MA, Mathews CE, Phelps EA. Beta cell dysfunction occurs independently of insulitis in type 1 diabetes pathogenesis. Cell Rep. 2025 Sep 23;44(9):116174. doi: 10.1016/j.celrep.2025.116174. Epub 2025 Aug 27. PMID: 40875294; PMCID: PMC12550822.
- Islet calcium imaging analysis code https://github.com/PhelpsLabUF/Ferreira-et-al.-2025
- Related paper: Ferreira SM, Hagan DW, Stis AE, Widener AE, Cuaycal AE, Rancourt C, Readey AG, Smurlick DS, Fu DA, Campbell-Thompson M, Rupnik MS, Phelps EA. Beta cell secreted GABA sets appropriate insulin secretion by modulating islet calcium oscillations. Mol Metab. 2025 Dec;102:102268. doi: 10.1016/j.molmet.2025.102268. Epub 2025 Oct 15. PMID: 41106726; PMCID: PMC12603775.
Perifusion
- Islet perifusion data analysis code https://github.com/PhelpsLabUF/Perifusion_data_analysis
- Related paper: Huber MK, Widener AE, Cuaycal AE, Smurlick D, Butterworth EA, Lenchik NI, Chen J, Beery M, Hiller H, Verney E, Kusmartseva I, Rupnik MS, Campbell-Thompson M, Gerling IC, Atkinson MA, Mathews CE, Phelps EA. Beta cell dysfunction occurs independently of insulitis in type 1 diabetes pathogenesis. Cell Rep. 2025 Sep 23;44(9):116174. doi: 10.1016/j.celrep.2025.116174. Epub 2025 Aug 27. PMID: 40875294; PMCID: PMC12550822.
Laser capture islet transcriptomics
- In collaboration with Clayton Mathews lab
- 260 islets gene expression dataframe: https://doi.org/10.5281/zenodo.14537115
- Analysis code: https://github.com/AlexandraCuaycal/laser_capture_microdissected_islet_transcriptomics
- Shiny app to explore data: https://alexandracuaycal.shinyapps.io/shiny_apps/
- Related paper: Huber MK, Widener AE, Cuaycal AE, Smurlick D, Butterworth EA, Lenchik NI, Chen J, Beery M, Hiller H, Verney E, Kusmartseva I, Rupnik MS, Campbell-Thompson M, Gerling IC, Atkinson MA, Mathews CE, Phelps EA. Beta cell dysfunction occurs independently of insulitis in type 1 diabetes pathogenesis. Cell Rep. 2025 Sep 23;44(9):116174. doi: 10.1016/j.celrep.2025.116174. Epub 2025 Aug 27. PMID: 40875294; PMCID: PMC12550822.
- Related paper: Cuaycal AE, Butterworth EA, Stimpson S, Chen J, Lenchik NI, Baratta LA, Phelps EA, Grieshaber S, Atkinson MA, Qian W-J, Campbell-Thompson M, Gerling IC, Mathews CE. Mitochondrial and protein homeostasis pathways are transcriptionally impaired in islets during type 1 diabetes pathogenesis. bioRxiv. 2026. doi: 10.64898/2026.07.29.741469.
