{"id":4561,"date":"2025-05-19T08:43:13","date_gmt":"2025-05-19T12:43:13","guid":{"rendered":"https:\/\/faculty.eng.ufl.edu\/stabler\/?page_id=4561"},"modified":"2025-11-20T15:11:26","modified_gmt":"2025-11-20T19:11:26","slug":"ultrathin-coatings","status":"publish","type":"page","link":"https:\/\/faculty.eng.ufl.edu\/stabler\/research\/ultrathin-coatings\/","title":{"rendered":"Ultrathin Coatings"},"content":{"rendered":"\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"990\" height=\"420\" src=\"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-content\/uploads\/sites\/497\/2015\/05\/TOC-REVISED-2.jpg\" alt=\"\" class=\"wp-image-103\" srcset=\"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-content\/uploads\/sites\/497\/2015\/05\/TOC-REVISED-2.jpg 990w, https:\/\/faculty.eng.ufl.edu\/stabler\/wp-content\/uploads\/sites\/497\/2015\/05\/TOC-REVISED-2-300x127.jpg 300w, https:\/\/faculty.eng.ufl.edu\/stabler\/wp-content\/uploads\/sites\/497\/2015\/05\/TOC-REVISED-2-768x326.jpg 768w\" sizes=\"auto, (max-width: 990px) 100vw, 990px\" \/><\/figure>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>The encapsulation\/immunoisolation of cells has numerous applications in cellular transplantation, particularly for diabetes. Conventional encapsulation methods impose consequential mass transport limitations and transplant volumes. New approaches, such as layer-by-layer encapsulation, generate ultrathin coatings, but common challenges include incomplete encapsulation, coating stability, and method cytotoxicity.<\/p>\n\n\n\n<p>In our patented approach, we have used functionalized polymers capable of forming stable, covalent linkages via Staudinger ligation\u2014a spontaneous, chemoselective, and cell-compatible reaction. We have subsequently explored the use of these complementary PEG- and alginate-based polymers to fabricate covalently linked, nano-thick coatings on cellular spheroids, particularly islets. We have found these coatings to be highly cell compatible, with no impairment of glucose responsiveness.<\/p>\n\n\n\n<p>Once coated, these polymers can also serve as a platform for the tethering of additional agents, which would subsequently serve to instruct the surrounding host environment or support islet engraftment.<\/p>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h6 class=\"wp-block-heading has-text-align-center\">These efforts are currently being supported by an <a href=\"https:\/\/projectreporter.nih.gov\/project_info_description.cfm?aid=10142609&amp;icde=53151316\" data-type=\"link\" data-id=\"https:\/\/projectreporter.nih.gov\/project_info_description.cfm?aid=10142609&amp;icde=53151316\">NIH R01 grant.<\/a><\/h6>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The encapsulation\/immunoisolation of cells has numerous applications in cellular transplantation, particularly for diabetes. Conventional encapsulation methods impose consequential mass transport limitations and transplant volumes. New approaches, such as layer-by-layer encapsulation, generate ultrathin coatings, but common challenges include incomplete encapsulation, coating stability, and method cytotoxicity. In our patented approach, we have used functionalized polymers capable of [&hellip;]<\/p>\n","protected":false},"author":1183,"featured_media":0,"parent":84,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-section-nav.php","meta":{"_acf_changed":false,"inline_featured_image":false,"featured_post":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-4561","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-json\/wp\/v2\/pages\/4561","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-json\/wp\/v2\/users\/1183"}],"replies":[{"embeddable":true,"href":"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-json\/wp\/v2\/comments?post=4561"}],"version-history":[{"count":9,"href":"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-json\/wp\/v2\/pages\/4561\/revisions"}],"predecessor-version":[{"id":5035,"href":"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-json\/wp\/v2\/pages\/4561\/revisions\/5035"}],"up":[{"embeddable":true,"href":"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-json\/wp\/v2\/pages\/84"}],"wp:attachment":[{"href":"https:\/\/faculty.eng.ufl.edu\/stabler\/wp-json\/wp\/v2\/media?parent=4561"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}