Skip to main content
Chemical Engineering Department College of Engineering

Facilities

Our lab’s expertise in chemically modifying and testing nucleic acids, peptides, and proteins intersects chemistry, biology, nanotechnology and genomics.

  • Chemistry: Chemical modification, purification, and characterization of peptides, oligonucleotides, polymers, and small molecules. Jain lab has a dedicated chemistry suite with a hood and equipment.
    • We own a state-of-the-art microwave-assisted peptide synthesizer (CEM’s Liberty Blue), a HPLC (Agilent 1100) for purification, a NanoDrop for UV/Vis (Thermo oneC), a fluorimeter with 384-well capabilities (Biotek), UV crosslinker, a large gravity oven, and various small equipment. Easy access to LC/MS/MS analysis in the proteomics core within the building.
  • Protein Engineering: Jain Lab has an entire pipeline for discovering and engineering proteins. Well equipped with bioinformatics pipeline, plasmid design and reconstruction, recombinant DNA, protein expression, and purification.
    • We have two FPLCs (GE Akta Pure and BioRad Biologic) with affinity, SEC, and IEC setups, multiple temperature-controlled shakers and centrifuges, incubators, autosonifier, and homogenizers within the lab. Several shared ultracentrifuges, a cold room, and a dark room on the same floor. Easy access to LC/MS, MALDI, monoclonal antibody production, and bio-layer interferometry as a part of the core facilities within the building. Access to NMR, circular dichroism, and X-ray crystallography on campus.
  • Nanotechnology: Jain lab is set up for developing and analyzing a variety of nanoparticles and liposomal formulations.
    • DLS/Zetasizer (coming soon). Easy access to NanoSight NS300 and various electron microscopes within the building. A range of nano-characterization equipment available at the Research Service Center on campus.
  • Liquid Handling: Fast and safe liquid transfer
    • We have an automated liquid handler (Opentron OT-2) with single and multichannel options, Electronic multichannel pipettes (Integra and Thermo) and various other pipettors for liquid dispensing.
  • Molecular Biology: Jain lab is set up for a variety of molecular biology techniques including qPCR, PCR, plasmid design, siRNA design, sgRNA design and screening, in vitro transcription, gel electrophoresis, Western blot, ELISA, and enzymatic assays
    • Two qPCR (Agilent and ViiA 7 systems) three PCR machines (Thermo 3-block, Eppendorf, and BioRad), an iWestern workflow system (Thermo), and several gel electrophoresis systems. We also own two gel imagers- a phosphorimager/chemiluminescent/fluorescence (GE Typhoon) and a RGB gel imager (Analytik Jena, GelStudio Touch Plus). A variety of liquid handling and small molecular biology equipment are also in the lab. 
  • Tissue Culture: Jain lab is equipped with mammalian cell culture, transfection, transduction, transformation, electroporation, viral production, and microscopy.
    • Dedicated BL2+ tissue culture suite with two large incubators, two 4-ft hoods, two large centrifuges in the room, a fluorescence digital inverted microscope with four color channels (EVOS), an electroporator (Neon), and an automated fluorescence cell counter (Countess). Access to various fluorescence, multiphoton, and confocal microscopes, flow cytometry, cell sorting, and histology core facilities within the building.
  • Genomics: Genome editing with CRISPR/Cas9, mutagenesis and mutation detection, DNA isolation, Sequencing, DNA library preparation for NGS.
    • A shared pyrosequencer. Immediate access to various RNASeq and NGS equipment. Bioinformatics and DNA sequencing core facilities within the building.
  • Animals: Currently animal research has not yet started in the lab. However, Dr. Jain has experience in mice breeding, genotyping, tagging, injections, surgeries, perfusion, organ isolation, magnetic cell isolation, IVIS imaging, and euthanasia.
    • Jain Lab owns a Miltenyi’s autoMACS Pro Separator, an isoflurane device, and a tissue homogenizer (coming soon). The Jain lab is located in the Cancer and Genetics Research Complex, which has an animal facility within the building. 
  • Computer and software: Dedicated lab server and a computer for individuals and equipment in the lab (>10 total). Software for bioinformatics, plasmid design, molecular modeling, graphical design, experimental design, and statistical analysis are available in the lab.